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Modulazione da analisi di inibitore HDAC genoma di microRNA e mRNAs in B le cellule indotte a sottoporsi switch classe DNA ricombinazione e differenziazione delle cellule di Plasma
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Immunology and Infection
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JoVE Journal Immunology and Infection
Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation
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Modulazione da analisi di inibitore HDAC genoma di microRNA e mRNAs in B le cellule indotte a sottoporsi switch classe DNA ricombinazione e differenziazione delle cellule di Plasma

italiano

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11:06 min

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September 20, 2017

DOI:

10.3791/55135-v

DOI:
10.3791/55135-v

•

11:06 min
September 20, 2017

•

3 Views
, , , , ,
1Department of Microbiology, Immunology and Molecular Genetics,University of Texas Long School of Medicine at San Antonio, 2Xiangya School of Medicine,Cental South University, 3Greehey Children’s Cancer Research Institute,University of Texas Long School of Medicine at San Antonio

Summary

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I fattori epigenetici possono interagire con programmi genetici di modulare l'espressione genica e regolare la funzione delle cellule B. Combinando la stimolazione in vitro delle cellule di B, qRT-PCR e microRNA-sequenza di alto-rendimento e sequenza di mRNA approcci, possiamo analizzare la modulazione epigenetica di miRNA e l'espressione genica in cellule di B.

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Cite this Article

Sanchez, H. N., Shen, T., Garcia, D., Lai, Z., Casali, P., Zan, H. Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation. J. Vis. Exp. (127), e55135, doi:10.3791/55135 (2017).

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