Journal
/
Imunologia e Infecção
/
Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

DOI:
10.3791/55135-v

•

11:06 min

•

September 20, 2017

•

, , , , ,
1Department of Microbiology, Immunology and Molecular Genetics,University of Texas Long School of Medicine at San Antonio, 2Xiangya School of Medicine,Cental South University, 3Greehey Children’s Cancer Research Institute,University of Texas Long School of Medicine at San Antonio

Capítulos

  • 00:05Título
  • 00:56Stimulation of Mouse B Cells for Class-switch DNA Recombination (CSR), Plasma Differentiation and HDI Treatment
  • 03:04B-cell Isolation
  • 04:26B-cell Stimulation and HDI Treatment
  • 05:17Flow Cytometry Analysis of CSR and Plasma Cell Differentiation
  • 07:11Quantitative RT-PCR of mRNA and miRNAs
  • 08:41Results: Analysis of Epigenetic Modulation of miRNA and Gene Expression in B Cells
  • 10:28Conclusion

Summary

Tadução automática

Tadução automática

Epigenetic factors can interact with genetic programs to modulate gene expression and regulate B cell function. By combining in vitro B-cell stimulation, qRT-PCR, and high-throughput microRNA-sequence and mRNA-sequence approaches, we can analyze the epigenetic modulation of miRNA and gene expression in B cells.

Tags

Keywords: Genome-wide AnalysisHDAC InhibitorMicroRNAMRNAB CellsClass-switch DNA RecombinationPlasma Cell DifferentiationEpigenetic RegulationAntibody ResponseImmunoglobulin Class-switchingCell IsolationSpleenCell CultureCell CountingFlow Cytometry

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/55135?article_type=v

Induction and Assessment of Class Switch Recombination in Purified Murine B Cells
check_url/pt/55135?article_type=v

Directed Differentiation of Induced Pluripotent Stem Cells towards T Lymphocytes
check_url/pt/55135?article_type=v

Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages
check_url/pt/55135?article_type=v

Rapid Molecular Detection and Differentiation of Influenza Viruses A and B
check_url/pt/55135?article_type=v

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity
check_url/pt/55135?article_type=v

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
check_url/pt/55135?article_type=v

An In Vivo Mouse Model to Measure Naïve CD4 T Cell Activation, Proliferation and Th1 Differentiation Induced by Bone Marrow-derived Dendritic Cells
check_url/pt/55135?article_type=v

Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro
check_url/pt/55135?article_type=v

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
check_url/pt/55135?article_type=v

Studying Organelle Dynamics in B Cells During Immune Synapse Formation

Read Article