Journal
/
Immunologie und Infektion
/
Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation
JoVE Journal
Immunologie und Infektion
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Immunologie und Infektion
Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

6,043 Views

•

11:06 min

•

September 20, 2017

DOI:

10.3791/55135-v

DOI:
10.3791/55135-v

•

11:06 min
September 20, 2017

•

3 Views
, , , , ,
1Department of Microbiology, Immunology and Molecular Genetics,University of Texas Long School of Medicine at San Antonio, 2Xiangya School of Medicine,Cental South University, 3Greehey Children’s Cancer Research Institute,University of Texas Long School of Medicine at San Antonio

Summary

Automatically generated

Epigenetic factors can interact with genetic programs to modulate gene expression and regulate B cell function. By combining in vitro B-cell stimulation, qRT-PCR, and high-throughput microRNA-sequence and mRNA-sequence approaches, we can analyze the epigenetic modulation of miRNA and gene expression in B cells.

Explore More Videos

Genome-wide AnalysisHDAC InhibitorMicroRNAMRNAB CellsClass-switch DNA RecombinationPlasma Cell DifferentiationEpigenetic RegulationAntibody ResponseImmunoglobulin Class-switchingCell IsolationSpleenCell CultureCell CountingFlow Cytometry

Read Article

Cite this Article

Sanchez, H. N., Shen, T., Garcia, D., Lai, Z., Casali, P., Zan, H. Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation. J. Vis. Exp. (127), e55135, doi:10.3791/55135 (2017).

Download .ris file

Copy

Share Video

.

Copy