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低周波体細胞変異の検出のための高感度な方法として直接配列決定と組み合わせるPCRをブロックする野生型
JoVE Journal
Genetics
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JoVE Journal Genetics
Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

低周波体細胞変異の検出のための高感度な方法として直接配列決定と組み合わせるPCRをブロックする野生型

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10:41 min

March 29, 2017

DOI:

10:41 min
March 29, 2017

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Summary

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Wild-type blocking PCR followed by direct sequencing offers a highly sensitive method of detection for low frequency somatic mutations in a variety of sample types.

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