Journal
/
Genética
/
Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
JoVE Journal
Genética
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Genética
Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

11,385 Views

•

10:41 min

•

March 29, 2017

DOI:

10.3791/55130-v

DOI:
10.3791/55130-v

•

10:41 min
March 29, 2017

•

1 Views
, ,
1NeoGenomics Laboratories

Tags

Keyword Extraction:Wild-type Blocking PCRSomatic MutationsMyd88 GeneBone Marrow SamplesExon FiveL265 HotspotM13 SequenceBlocking OligonucleotideWild-type TemplateMelting TemperatureExtension TemperatureSecondary StructureSelf-dimerization

Read Article

Cite this Article

Albitar, A. Z., Ma, W., Albitar, M. Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations. J. Vis. Exp. (121), e55130, doi:10.3791/55130 (2017).

Download .ris file

Copy

Share Video

.

Copy