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En enkel ett-trinns Dissection Protokoll for Whole-mount Utarbeidelse av Adult<em> Drosophila</em> Brains
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1Center for Metabolic and Degenerative Diseases, The Brown Foundation Institute of Molecular Medicine, Programs in Human and Molecular Genetics and Neuroscience, The Graduate School of Biomedical Sciences at Houston,The University of Texas Health Science Center at Houston, 2Department of Natural Sciences,University of Houston – Downtown, 3Department of Neurobiology and Anatomy, McGovern Medical School,The University of Texas Health Science Center at Houston
Chapters
- 00:05Title
- 01:12Microsurgical Dissection of Adult Fly Heads
- 02:56Immunofluorescent Staining of the Brains
- 04:44Mounting and Imaging of Brain Tissue
- 06:02Results: Anti-TH Staining in Dissected Fly Brains Allows Quantification of DA Neurons
- 07:40Conclusion
Den voksne Drosophila hjernen er et verdifullt system for å studere nevrale kretser, høyere hjernefunksjoner, og sammensatte lidelser. En effektiv metode for å dissekere hele hjernevev fra den lille flua hodet vil lette hjernebaserte studier. Her beskriver vi en enkel, ett-trinns disseksjon protokoll av voksne hjerner med godt bevarte morfologi.
