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A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis

JoVE Journal
生化学

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JoVE Journal 生化学

A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis

A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis

8,193 Views

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05:43 min

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January 24, 2017

DOI:

10.3791/55077-v

DOI:

10.3791/55077-v

•

05:43 min

January 24, 2017

•

3 Views

Megumi Tsuchiya*¹, M. Rezaul Karim*², Taro Matsumoto³, Hidesato Ogawa¹, Hiroaki Taniguchi^3,4,5

¹Graduate School of Frontier Biosciences,Osaka University, ²Department of Biotechnology and Genetic Engineering,Jahangirnagar University, ³Division of Cell Regeneration and Transplantation,School of Medicine, Nihon University, ⁴Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, ⁵Graduate School of Life and Medical Sciences,Doshisha University

概要

We have established a method for the purification of coregulatory interaction proteins using the LC-MS/MS system.

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