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Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells
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January 15, 2018
DOI:
1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute,The Blood Center of Wisconsin, 2Vector Core Lab, Blood Research Institute,The Blood Center of Wisconsin, 3Laboratory of Lymphocyte Biology, Blood Research Institute,The Blood Center of Wisconsin, 4Laboratory of Stem Cell Transcriptional Regulation, Blood Research Institute,The Blood Center of Wisconsin, 5Department of Microbiology and Immunology,The Medical College of Wisconsin, 6小児科,The Medical College of Wisconsin, 7医学部,The Medical College of Wisconsin
概要
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The goal of this study was to formulate technologies that allow for successful gene transduction in primary natural killer (NK) cells. The dextran-mediated lentiviral transduction of human or mouse primary NK cells results in higher gene expression efficiencies. This method of gene transduction will vastly improve NK cell genetic manipulation.
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Nanbakhsh, A., Best, B., Riese, M., Rao, S., Wang, L., Medin, J., Thakar, M. S., Malarkannan, S. Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells. J. Vis. Exp. (131), e55063, doi:10.3791/55063 (2018).
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