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死後ヒト脳ドナーから軟膜植片培養物の導出
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Neuroscience
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JoVE Journal Neuroscience
Derivation of Leptomeninges Explant Cultures from Postmortem Human Brain Donors
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死後ヒト脳ドナーから軟膜植片培養物の導出

日本語

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8,089 Views

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05:18 min

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January 21, 2017

DOI:

10.3791/55045-v

DOI:
10.3791/55045-v

•

05:18 min
January 21, 2017

•

7 Views
, , , ,
1Parkinson’s Institute and Clinical Center

Summary

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人間の死後脳から軟膜外植片培養プロトコルは6-8週間以内にフィブロネクチン陽性髄膜線維芽細胞を導出し、約20から30000000細胞を凍結保存するための技術的に堅牢かつ簡単な方法です。

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Cite this Article

Lee, K., Saetern, O. C., Nguyen, A., Rodriguez, L., Schüle, B. Derivation of Leptomeninges Explant Cultures from Postmortem Human Brain Donors. J. Vis. Exp. (119), e55045, doi:10.3791/55045 (2017).

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