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Derivation of Leptomeninges Explant Cultures from Postmortem Human Brain Donors
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January 21, 2017
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The leptomeninges explant culture protocol from human postmortem brain is a technically robust and simple way to derive fibronectin-positive meningeal fibroblasts within 6-8 weeks and cryopreserve approximately 20-30 million cells.
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Lee, K., Saetern, O. C., Nguyen, A., Rodriguez, L., Schüle, B. Derivation of Leptomeninges Explant Cultures from Postmortem Human Brain Donors. J. Vis. Exp. (119), e55045, doi:10.3791/55045 (2017).
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