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Un metodo efficace per l'isolamento di RNA altamente purificato da semi per l'utilizzo in Quantitative Analysis trascrittoma
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Biochemistry
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JoVE Journal Biochemistry
An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
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Un metodo efficace per l'isolamento di RNA altamente purificato da semi per l'utilizzo in Quantitative Analysis trascrittoma

italiano

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9,444 Views

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06:31 min

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January 11, 2017

DOI:

10.3791/55008-v

DOI:
10.3791/55008-v

•

06:31 min
January 11, 2017

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3 Views
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1Laboratory of Biological Diversity, Department of Evolutionary Biology and Biodiversity,National Institute for Basic Biology, 2Department of Basic Biology,SOKENDAI (The Graduate University for Advanced Studies), 3Department of Cell Biology,National Institute for Basic Biology

Summary

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We have succeeded in establishing a method for RNA isolation from plant seeds containing large amounts of oils, proteins, and polyphenols, which have inhibitory effects on high-purity RNA isolation. Our method is suitable for monitoring the expression of genes with low level transcripts in seeds.

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Kanai, M., Mano, S., Nishimura, M. An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis. J. Vis. Exp. (119), e55008, doi:10.3791/55008 (2017).

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