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Visualizando o Tissue interrenais Steroidogenic e sua Vascular Microenvironment em Zebrafish
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07:19 min
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December 21, 2016
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Summary
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A glândula interrenal no peixe-zebra é a contrapartida teleósteos da glândula supra-renal de mamífero. Este protocolo apresenta a forma de realizar a desidrogenase de 3-hidroxiesteróides β (Δ 5-4 isomerase; 3β-HSD) ensaio da actividade enzimática, que detecta as células diferenciadas esteroidogênicas no peixe-zebra desenvolvimento.
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Chou, C., Lin, J., Hou, H., Liu, Y. Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish. J. Vis. Exp. (118), e54820, doi:10.3791/54820 (2016).
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