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Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish

Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish

DOI:
10.3791/54820-v

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07:19 min

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December 21, 2016

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, , ,
1Department of Life Science,Tunghai University

Bölümler

  • 00:05Başlık
  • 01:04Whole Mount 3β-Hsd Activity Staining
  • 03:15IHC Analysis of 3β-Hsd Activity-stained Embryos
  • 05:28Results: Zebrafish Interrenal Steroidogenic Tissue
  • 06:30Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

The interrenal gland in zebrafish is the teleostean counterpart of the mammalian adrenal gland. This protocol introduces how to perform the 3-β-hydroxysteroid dehydrogenase (Δ5-4 isomerase; 3β-Hsd) enzymatic activity assay, which detects differentiated steroidogenic cells in the developing zebrafish.

Etiketler

Vascular MicroenvironmentZebrafishEnzymatic Activity AssayWhole-mount StainingTransgenic Fluorescent Reporter LinesVibratome SectioningImmunohistochemistryConfocal Microscopy3beta-Hsd Activity StainingPigment InhibitionPFA FixationPBST WashingChromogenic Signal DetectionPrecipitationPost-fixationGlycerol ClearingWhole-mount Microscopy

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