/
/
Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish
6,801 Views
•
07:19 min
•
December 21, 2016
DOI:
Summary
Automatically generated
The interrenal gland in zebrafish is the teleostean counterpart of the mammalian adrenal gland. This protocol introduces how to perform the 3-β-hydroxysteroid dehydrogenase (Δ5-4 isomerase; 3β-Hsd) enzymatic activity assay, which detects differentiated steroidogenic cells in the developing zebrafish.
Explore More Videos
Vascular MicroenvironmentZebrafishEnzymatic Activity AssayWhole-mount StainingTransgenic Fluorescent Reporter LinesVibratome SectioningImmunohistochemistryConfocal Microscopy3beta-Hsd Activity StainingPigment InhibitionPFA FixationPBST WashingChromogenic Signal DetectionPrecipitationPost-fixationGlycerol ClearingWhole-mount Microscopy
Read Article
Cite this Article
Chou, C., Lin, J., Hou, H., Liu, Y. Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish. J. Vis. Exp. (118), e54820, doi:10.3791/54820 (2016).
Copy