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Histon-miniSOG Kullanılan optogenetic rastgele mutagenez<em> C. elegans</em
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Genetics
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JoVE Journal Genetics
Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
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Histon-miniSOG Kullanılan optogenetic rastgele mutagenez<em> C. elegans</em

Türkçe

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8,880 Views

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04:51 min

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November 14, 2016

DOI:

10.3791/54810-v

DOI:
10.3791/54810-v

•

04:51 min
November 14, 2016

•

2 Views
,
1Division of Biological Sciences, Section of Neurobiology,University of California in San Diego and Howard Hughes Medical Institute, 2Department of Cellular and Molecular Medicine,University of California in San Diego School of Medicine and Howard Hughes Medical Institute

Summary

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Genetik olarak kodlanmış histon miniSOG mavi ışığa bağımlı bir şekilde genom kalıtsal mutasyonlara neden olur. Bu mutajenez yöntemi, hızlı, basit toksik kimyasalların serbest ve ileri genetik tarama ve transgen entegrasyonu için çok uygundur.

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Cite this Article

Noma, K., Jin, Y. Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans. J. Vis. Exp. (117), e54810, doi:10.3791/54810 (2016).

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