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La detección de alto rendimiento de los patógenos respiratorios de especímenes animales por PCR nanoescala
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
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La detección de alto rendimiento de los patógenos respiratorios de especímenes animales por PCR nanoescala

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11,651 Views

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11:00 min

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November 28, 2016

DOI:

10.3791/54781-v

DOI:
10.3791/54781-v

•

11:00 min
November 28, 2016

•

2 Views
, , , , , , , , ,
1Population Medicine and Diagnostic Sciences,Cornell University Animal Health Diagnostic Center, 2Thermo Fisher Scientific Inc.

Summary

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pruebas de alto rendimiento de ADN y ARN patógenos basados ​​por nanoescala PCR se describe el uso de un canino sindrómica y el panel de PCR respiratoria equina.

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Keyword Extraction:High-throughput DetectionRespiratory PathogensAnimal SpecimensNanoscale PCRReal-time PCRDNARNAPathogensOneHealth SurveillanceInfectious DiseasesClinical SpecimensRespiratory PathogensHorsesDogsLysis BufferReverse TranscriptionPre-amplificationPreamp96-well PlateThermocyclerCDNA Production

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Goodman, L. B., Anderson, R. R., Slater, M., Ortenberg, E., Renshaw, R. W., Chilson, B. D., Laverack, M. A., Beeby, J. S., Dubovi, E. J., Glaser, A. L. High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR. J. Vis. Exp. (117), e54781, doi:10.3791/54781 (2016).

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