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High-throughput detectie van respiratoire pathogene in dierlijke specimens door nanoschaal PCR
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
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High-throughput detectie van respiratoire pathogene in dierlijke specimens door nanoschaal PCR

Nederlands

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11,651 Views

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11:00 min

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November 28, 2016

DOI:

10.3791/54781-v

DOI:
10.3791/54781-v

•

11:00 min
November 28, 2016

•

2 Views
, , , , , , , , ,
1Population Medicine and Diagnostic Sciences,Cornell University Animal Health Diagnostic Center, 2Thermo Fisher Scientific Inc.

Summary

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High-throughput testen van DNA en RNA gebaseerde pathogenen op nanoschaal PCR wordt omschreven met een syndromale honden en paarden luchtwegen PCR panel.

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Keyword Extraction:High-throughput DetectionRespiratory PathogensAnimal SpecimensNanoscale PCRReal-time PCRDNARNAPathogensOneHealth SurveillanceInfectious DiseasesClinical SpecimensRespiratory PathogensHorsesDogsLysis BufferReverse TranscriptionPre-amplificationPreamp96-well PlateThermocyclerCDNA Production

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Cite this Article

Goodman, L. B., Anderson, R. R., Slater, M., Ortenberg, E., Renshaw, R. W., Chilson, B. D., Laverack, M. A., Beeby, J. S., Dubovi, E. J., Glaser, A. L. High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR. J. Vis. Exp. (117), e54781, doi:10.3791/54781 (2016).

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