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Produzione semplici ed efficaci e Purificazione di topo mielina Oligodendrocyte glicoproteina di encefalomielite autoimmune sperimentale Studi
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Simple and Efficient Production and Purification of Mouse Myelin Oligodendrocyte Glycoprotein for Experimental Autoimmune Encephalomyelitis Studies

Produzione semplici ed efficaci e Purificazione di topo mielina Oligodendrocyte glicoproteina di encefalomielite autoimmune sperimentale Studi

10,750 Views

10:47 min

October 27, 2016

DOI:

10:47 min
October 27, 2016

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Summary

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We describe a simple protocol using only basic lab equipment to generate and purify large quantities of a fusion protein that contains mouse Myelin Oligodendrocyte Glycoprotein. This protein can be used to induce experimental autoimmune encephalomyelitis driven by both T and B cells.

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