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Immunology and Infection

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JoVE Journal Immunology and Infection
Simple and Efficient Production and Purification of Mouse Myelin Oligodendrocyte Glycoprotein for Experimental Autoimmune Encephalomyelitis Studies
 

Simple and Efficient Production and Purification of Mouse Myelin Oligodendrocyte Glycoprotein for Experimental Autoimmune Encephalomyelitis Studies

Article DOI: 10.3791/54727-v • 10:47 min • October 27th, 2016
October 27th, 2016 •
1, 1, 1
1Department of Microbiology and Immunology, Western University

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Resumo
Automatic Translation

We describe a simple protocol using only basic lab equipment to generate and purify large quantities of a fusion protein that contains mouse Myelin Oligodendrocyte Glycoprotein. This protein can be used to induce experimental autoimmune encephalomyelitis driven by both T and B cells.

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Keywords: Mouse Myelin Oligodendrocyte Glycoprotein Experimental Autoimmune Encephalomyelitis MOG Protein Protein Expression BL21 E. Coli IPTG Induction Cell Lysis Protein Purification Hexa-histidine Tag Nickel-nitrilotriacetic Acid (Ni-NTA) Affinity Chromatography
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