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Simple and Efficient Production and Purification of Mouse Myelin Oligodendrocyte Glycoprotein for Experimental Autoimmune Encephalomyelitis Studies
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Simple and Efficient Production and Purification of Mouse Myelin Oligodendrocyte Glycoprotein for Experimental Autoimmune Encephalomyelitis Studies
Bölümler
- 00:05Başlık
- 00:54Protein Induction
- 02:03Harvesting MOGtag Protein
- 03:42Protein Purification
- 06:17Dialysis and Concentrating MOGtag Protein
- 08:58Results: Evaluation of MOGtag Purity and Assessment of its Activity
- 09:39Conclusion
We describe a simple protocol using only basic lab equipment to generate and purify large quantities of a fusion protein that contains mouse Myelin Oligodendrocyte Glycoprotein. This protein can be used to induce experimental autoimmune encephalomyelitis driven by both T and B cells.
