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Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain

8,312 Views

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08:48 min

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October 25, 2016

DOI:

10.3791/54697-v

DOI:
10.3791/54697-v

•

08:48 min
October 25, 2016

•

5 Views
, , , , , , , , ,
1Department of Immunology,University of Toronto, 2Princess Margaret Cancer Centre,University Health Network

Tags

De Novo T Cell ReceptorTCR Hemi-chainRetroviral TransductionAntigen-specific T CellsGene EngineeringImmunotherapyPCR CloningBacterial TransformationPlasmid Purification293GPG Cell TransfectionPG-13 Packaging Cell LinePBMC Activation

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Guo, T., Ochi, T., Nakatsugawa, M., Kagoya, Y., Anczurowski, M., Wang, C., Rahman, M. A., Saso, K., Butler, M. O., Hirano, N. Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain. J. Vis. Exp. (116), e54697, doi:10.3791/54697 (2016).

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