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Hazırlama ve<em> İn Vivo</em> Bir Faaliyet tabanlı Sondası kullanılması için<em> K</em> -acylethanolamine Asit aminidaz
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Biochemistry
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JoVE Journal Biochemistry
Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
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Hazırlama ve<em> İn Vivo</em> Bir Faaliyet tabanlı Sondası kullanılması için<em> K</em> -acylethanolamine Asit aminidaz

Türkçe

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9,516 Views

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11:01 min

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November 23, 2016

DOI:

10.3791/54652-v

DOI:
10.3791/54652-v

•

11:01 min
November 23, 2016

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1 Views
, , , , , , ,
1Drug Discovery and Development,Istituto Italiano di Tecnologia, 2Departments of Anatomy and Neurobiology, Pharmacology, and Biological Chemistry,University of California, Irvine School of Medicine

Summary

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Burada, bir etkinlik bazlı prob hazırlanmasını ve kullanımını tarif (ARN14686, undek-10-inil-N - [(3S) -2-oxoazetidin-3-il] karbamat) saptanması ve ölçülmesi için izin verir proinflamatuar enzimi N -acylethanolamine asit amidaz aktif formu (NAAA), hem in vitro ve ex vivo.

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Romeo, E., Pontis, S., Ponzano, S., Bonezzi, F., Migliore, M., Di Martino, S., Summa, M., Piomelli, D. Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase. J. Vis. Exp. (117), e54652, doi:10.3791/54652 (2016).

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