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preparação e<em> In Vivo</em> O uso de uma sonda baseado em atividades para<em> N</em> Amidase Ácido -acylethanolamine
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Biochemistry
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JoVE Journal Biochemistry
Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

preparação e<em> In Vivo</em> O uso de uma sonda baseado em atividades para<em> N</em> Amidase Ácido -acylethanolamine

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11:01 min

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November 23, 2016

DOI:

10.3791/54652-v

DOI:
10.3791/54652-v

•

11:01 min
November 23, 2016

•

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1Drug Discovery and Development,Istituto Italiano di Tecnologia, 2Departments of Anatomy and Neurobiology, Pharmacology, and Biological Chemistry,University of California, Irvine School of Medicine

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Activity-based ProbeN-acylethanolamine Acid Amidase (NAAA)SynthesisIn Vivo UseFluorescence MicroscopyInflammatory DiseasesNeurodegenerative DiseasesUndecynenolDMAPDPCDichloromethaneSodium BicarbonateSodium SulfateOxo Asetydenol Ammonium AsetateDiazapropalethylamine

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Romeo, E., Pontis, S., Ponzano, S., Bonezzi, F., Migliore, M., Di Martino, S., Summa, M., Piomelli, D. Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase. J. Vis. Exp. (117), e54652, doi:10.3791/54652 (2016).

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