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Preparazione e<em> In Vivo</em> L'utilizzo di una sonda Activity-based per<em> N</em> -acylethanolamine Acid amidasi
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Biochemistry
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JoVE Journal Biochemistry
Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
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Preparazione e<em> In Vivo</em> L'utilizzo di una sonda Activity-based per<em> N</em> -acylethanolamine Acid amidasi

italiano

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9,516 Views

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11:01 min

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November 23, 2016

DOI:

10.3791/54652-v

DOI:
10.3791/54652-v

•

11:01 min
November 23, 2016

•

1 Views
, , , , , , ,
1Drug Discovery and Development,Istituto Italiano di Tecnologia, 2Departments of Anatomy and Neurobiology, Pharmacology, and Biological Chemistry,University of California, Irvine School of Medicine

Summary

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Qui, descriviamo la preparazione e l'uso di una sonda per attività (ARN14686, undec-10-ynyl- N - [(3 S) -2-oxoazetidin-3-il] carbammato) che consente la rilevazione e quantificazione del forma attiva dell'enzima proinfiammatorie N amidasica acido -acylethanolamine (NAAA), sia in vitro ed ex vivo.

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Cite this Article

Romeo, E., Pontis, S., Ponzano, S., Bonezzi, F., Migliore, M., Di Martino, S., Summa, M., Piomelli, D. Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase. J. Vis. Exp. (117), e54652, doi:10.3791/54652 (2016).

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