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Preparação de Matriz Extracelular fibras de proteína de Brillouin Spectroscopy
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy
DOI:

07:19 min

September 15, 2016

, , , , , ,

Chapters

  • 00:05Title
  • 00:46Rat Tail Collagen Fiber Extraction
  • 02:16Bovine Nuchal Ligament Elastin Fiber Extraction
  • 03:44Reflective Substrate Fiber Mounting, Brillouin Experiment Setup and Fiber Spectra Acquisition
  • 04:55Results: Representative Micromechanics of Collagen and Elastin Fibers
  • 06:50Conclusion

Summary

Automatic Translation

We present a protocol for the application of Brillouin light scattering spectroscopy to elastin and trypsin-purified type I collagen fibers of the extracellular matrix to extract their full elastic properties.

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