Journal
/
Bio-ingénierie
/
Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy
Journal JoVE
Bio-ingénierie
This content is Free Access.
Journal JoVE Bio-ingénierie
Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy

Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy

10,039 Views

•

07:19 min

•

September 15, 2016

DOI:

10.3791/54648-v

DOI:
10.3791/54648-v

•

07:19 min
September 15, 2016

•

5 Views
, , , , , ,
1School of Physics and Astronomy,University of Exeter, 2Department of Physics and Geology,University of Perugia, 3Istituto Officina dei Materiali del CNR,Unità di Perugia

Summary

Automatically generated

We present a protocol for the application of Brillouin light scattering spectroscopy to elastin and trypsin-purified type I collagen fibers of the extracellular matrix to extract their full elastic properties.

Explore More Videos

Extracellular MatrixCollagenElastinBrillouin SpectroscopyTendonChondroitinase ABCStreptomyces HyaluronidaseTrypsinBovine Nuchal LigamentSodium HydroxideWater Bath

Read Article

Cite this Article

Edginton, R. S., Mattana, S., Caponi, S., Fioretto, D., Green, E., Winlove, C. P., Palombo, F. Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy. J. Vis. Exp. (115), e54648, doi:10.3791/54648 (2016).

Download .ris file

Copy

Share Video

.

Copy