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Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy
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Biotechnik
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JoVE Journal Biotechnik
Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy

Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy

10,039 Views

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07:19 min

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September 15, 2016

DOI:

10.3791/54648-v

DOI:
10.3791/54648-v

•

07:19 min
September 15, 2016

•

5 Views
, , , , , ,
1School of Physics and Astronomy,University of Exeter, 2Department of Physics and Geology,University of Perugia, 3Istituto Officina dei Materiali del CNR,Unità di Perugia

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We present a protocol for the application of Brillouin light scattering spectroscopy to elastin and trypsin-purified type I collagen fibers of the extracellular matrix to extract their full elastic properties.

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Cite this Article

Edginton, R. S., Mattana, S., Caponi, S., Fioretto, D., Green, E., Winlove, C. P., Palombo, F. Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy. J. Vis. Exp. (115), e54648, doi:10.3791/54648 (2016).

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