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Clasificación de células activadas por fluorescencia para la purificación de células dendríticas plasmocitoide de la médula ósea de ratones
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Immunology and Infection
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JoVE Journal Immunology and Infection
Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow
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Clasificación de células activadas por fluorescencia para la purificación de células dendríticas plasmocitoide de la médula ósea de ratones

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11,820 Views

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08:01 min

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November 04, 2016

DOI:

10.3791/54641-v

DOI:
10.3791/54641-v

•

08:01 min
November 04, 2016

•

13 Views
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1Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine,Virginia Polytechnic Institute and State University, 2Flow Cytometry Laboratory, Virginia-Maryland College of Veterinary Medicine,Virginia Polytechnic Institute and State University

Summary

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Se presenta un protocolo mediante el uso de células activadas por fluorescencia para aislar las células dendríticas plasmacitoides (PDC) con alta pureza a partir de la médula ósea de ratones propensos al lupus para los estudios funcionales de PDC.

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Liao, X., Makris, M., Luo, X. M. Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow. J. Vis. Exp. (117), e54641, doi:10.3791/54641 (2016).

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