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Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

11,820 Views

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08:01 min

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November 04, 2016

DOI:

10.3791/54641-v

DOI:
10.3791/54641-v

•

08:01 min
November 04, 2016

•

13 Views
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1Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine,Virginia Polytechnic Institute and State University, 2Flow Cytometry Laboratory, Virginia-Maryland College of Veterinary Medicine,Virginia Polytechnic Institute and State University

Summary

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We report a protocol using fluorescence-activated cell sorting to isolate plasmacytoid dendritic cells (pDC) with high purity from the bone marrow of lupus-prone mice for functional studies of pDC.

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Liao, X., Makris, M., Luo, X. M. Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow. J. Vis. Exp. (117), e54641, doi:10.3791/54641 (2016).

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