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마우스 골수에서 형질 수지상 세포의 정화를위한 형광 활성화 셀 정렬
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Immunology and Infection
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JoVE Journal Immunology and Infection
Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow
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마우스 골수에서 형질 수지상 세포의 정화를위한 형광 활성화 셀 정렬

English

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11,779 Views

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08:01 min

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November 04, 2016

DOI:

10.3791/54641-v

DOI:
10.3791/54641-v

•

08:01 min
November 04, 2016

•

13 Views
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1Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine,Virginia Polytechnic Institute and State University, 2Flow Cytometry Laboratory, Virginia-Maryland College of Veterinary Medicine,Virginia Polytechnic Institute and State University

Summary

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우리는 PDC의 기능 연구를위한 루푸스 발생하기 쉬운 마우스의 골수로부터 고순도 형질 수지상 세포 (PDC)를 분리 정렬 형광 활성화 된 세포를 사용하여 프로토콜을보고합니다.

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Cite this Article

Liao, X., Makris, M., Luo, X. M. Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow. J. Vis. Exp. (117), e54641, doi:10.3791/54641 (2016).

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