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כריך נייר מסנן מתחת למים במשך לטווח ארוך<em> אקס אובו</em> זמן לשגות הדמיה של מוקדם צ'יק עובר
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December 28, 2016
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Here, we present a new technique to culture chick embryos ex ovo for time-lapse imaging using transmitted light and fluorescence microscopy. The submerged filter paper sandwich is a variant of the well-established filter paper carrier technique1 that allows for the culturing of chick embryos fully-submerged in a liquid culture medium.
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Schmitz, M., Nelemans, B. K. A., Smit, T. H. A Submerged Filter Paper Sandwich for Long-term Ex Ovo Time-lapse Imaging of Early Chick Embryos. J. Vis. Exp. (118), e54636, doi:10.3791/54636 (2016).
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