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A Submerged Filter Paper Sandwich for Long-term Ex Ovo Time-lapse Imaging of Early Chick Embryos
JoVE Journal
Gelişim Biyolojisi
This content is Free Access.
JoVE Journal Gelişim Biyolojisi
A Submerged Filter Paper Sandwich for Long-term Ex Ovo Time-lapse Imaging of Early Chick Embryos
DOI:

07:29 min

December 28, 2016

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'Bölümler'

  • 00:05'Başlık'
  • 00:37Submerged Filter Paper Sandwich Production
  • 05:11Results: Time-lapse Acquisition of Chick Embryonic Development
  • 06:48Conclusion

Özet

'Otomatik Çeviri'

Here, we present a new technique to culture chick embryos ex ovo for time-lapse imaging using transmitted light and fluorescence microscopy. The submerged filter paper sandwich is a variant of the well-established filter paper carrier technique1 that allows for the culturing of chick embryos fully-submerged in a liquid culture medium.

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