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Developmental Biology

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JoVE Journal Developmental Biology
A Submerged Filter Paper Sandwich for Long-term Ex Ovo Time-lapse Imaging of Early Chick Embryos
 

A Submerged Filter Paper Sandwich for Long-term Ex Ovo Time-lapse Imaging of Early Chick Embryos

Article DOI: 10.3791/54636-v • 07:29 min • December 28th, 2016
December 28th, 2016 •
*1, *1, 2
1Department of Orthopaedic Surgery, VU University Medical Center Amsterdam, MOVE Research Institute Amsterdam, 2Department of Anatomy, Embryology & Physiology, Academic Medical Center Amsterdam
* These authors contributed equally

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Here, we present a new technique to culture chick embryos ex ovo for time-lapse imaging using transmitted light and fluorescence microscopy. The submerged filter paper sandwich is a variant of the well-established filter paper carrier technique1 that allows for the culturing of chick embryos fully-submerged in a liquid culture medium.

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Keywords: Chick Embryos Ex Ovo Culture Time-lapse Imaging Neurulation Gastrulation Somitogenesis Pannett-Compton Saline Albumen Filter Paper Carrier Vitelline Membrane Yolk
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