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Estudo de Vetores Virais em um tridimensional do fígado Modelo repovoada com a linha de células humanas carcinoma hepatocelular HepG2
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2

Estudo de Vetores Virais em um tridimensional do fígado Modelo repovoada com a linha de células humanas carcinoma hepatocelular HepG2

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7,825 Views

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09:13 min

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October 24, 2016

DOI:

10.3791/54633-v

DOI:
10.3791/54633-v

•

09:13 min
October 24, 2016

•

4 Views
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1Department of Applied Biochemistry,Institute of Biotechnology, Berlin University of Technology, 2Department of Medical Biotechnology,Institute of Biotechnology, Berlin University of Technology, 3Department of Bioprocess Engineering,Institute of Biotechnology, Berlin University of Technology

Summary

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The recellularized extracellular matrix of a decellularized rat liver can be used as a humanized, three-dimensional ex vivo model to study the distribution and transgene expression of a virus or viral vector.

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Cite this Article

Hiller, T., Röhrs, V., Dehne, E., Wagner, A., Fechner, H., Lauster, R., Kurreck, J. Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2. J. Vis. Exp. (116), e54633, doi:10.3791/54633 (2016).

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