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Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2
JoVE Journal
がん研究
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JoVE Journal がん研究
Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2

Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2

DOI:
10.3791/54633-v

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09:13 min

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October 24, 2016

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, , , , , ,
1Department of Applied Biochemistry,Institute of Biotechnology, Berlin University of Technology, 2Department of Medical Biotechnology,Institute of Biotechnology, Berlin University of Technology, 3Department of Bioprocess Engineering,Institute of Biotechnology, Berlin University of Technology

章

  • 00:05標題
  • 01:08Recellularization of Extracellular Matrix (ECM) of a Rat Liver
  • 04:28Transduction of the Recellularized Rat Liver
  • 05:59Assessment of the Recellularized Transduced Rat Liver
  • 07:14Results: Recellularization Assessment and Transduction Efficiency
  • 08:39Conclusion

概要

自動翻訳

自動翻訳

The recellularized extracellular matrix of a decellularized rat liver can be used as a humanized, three-dimensional ex vivo model to study the distribution and transgene expression of a virus or viral vector.

タグ

3D Liver ModelHepG2 Cell LineViral VectorsGene TransferDecellularized Rat Liver ScaffoldBioreactor SystemRecellularizationAnimal WelfareCell CultureHepatocellular Carcinoma

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