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チックに誘導シグナルを研究するために、候補因子浸しビーズと組み合わせることで不浸透性の障壁の応用
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Application of Impermeable Barriers Combined with Candidate Factor Soaked Beads to Study Inductive Signals in the Chick
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チックに誘導シグナルを研究するために、候補因子浸しビーズと組み合わせることで不浸透性の障壁の応用

日本語

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9,158 Views

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08:04 min

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November 17, 2016

DOI:

10.3791/54618-v

DOI:
10.3791/54618-v

•

08:04 min
November 17, 2016

•

2 Views
,
1Randall Division of Cell and Molecular Biophysics,King’s College London, Guy’s Campus

Summary

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Protocols using impermeable barriers to block induction events between tissues of the main body axis and flank in the chick embryo required for limb formation are described. Beads soaked in candidate inductive signals are used to overcome the effect of barrier placement and analysis of gene expression confirms this.

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Cite this Article

Wilde, S., Logan, M. P. Application of Impermeable Barriers Combined with Candidate Factor Soaked Beads to Study Inductive Signals in the Chick. J. Vis. Exp. (117), e54618, doi:10.3791/54618 (2016).

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