Application des barrières imperméables combinées avec le facteur de candidat Perles Soaked pour étudier les signaux inductifs dans le poussin

Protocols using impermeable barriers to block induction events between tissues of the main body axis and flank in the chick embryo required for limb formation are described. Beads soaked in candidate inductive signals are used to overcome the effect of barrier placement and analysis of gene expression confirms this.