Application of Impermeable Barriers Combined with Candidate Factor Soaked Beads to Study Inductive Signals in the Chick

Susan Wilde; Malcolm P. Logan

doi:10.3791/54618

ジャーナル

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発生生物学

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Application of Impermeable Barriers Combined with Candidate Factor Soaked Beads to Study Inductive Signals in the Chick

JoVE Journal
発生生物学

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JoVE Journal 発生生物学

Application of Impermeable Barriers Combined with Candidate Factor Soaked Beads to Study Inductive Signals in the Chick

9,153 Views

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08:04 min

•

November 17, 2016

DOI:

10.3791/54618-v

DOI:

10.3791/54618-v

•

08:04 min

November 17, 2016

•

2 Views

Susan Wilde¹, Malcolm P. Logan¹

¹Randall Division of Cell and Molecular Biophysics,King’s College London, Guy’s Campus

概要

Protocols using impermeable barriers to block induction events between tissues of the main body axis and flank in the chick embryo required for limb formation are described. Beads soaked in candidate inductive signals are used to overcome the effect of barrier placement and analysis of gene expression confirms this.

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Application of Impermeable Barriers Combined with Candidate Factor Soaked Beads to Study Inductive Signals in the Chick

概要

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