/
/
La definición de Sustrato especificidades para la lipasa y la fosfolipasa candidatos
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:02Use Artificial Substrates for Optimizing Enzyme Activities of (Phospho)lipases
- 02:20In Vivo Identification of the Physiological Substrate of a Lipase
- 05:48In Vitro Identification of the Physiological Substrate of Diacylglycerol (DAG) Lipase
- 07:00Results: Predicted Phospholipase SMc01003 Acts on Artificial p-Nitrophenyl Acyl Ester and DAG
- 08:01Conclusion
Many predicted (phospho)lipases are poorly characterized with regard to their substrate specificities and physiological functions. Here we provide a protocol to optimize enzyme activities, search for natural substrates, and propose physiological functions for these enzymes.
