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Defining Substrate Specificities for Lipase and Phospholipase Candidates
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Biochimica
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JoVE Journal Biochimica
Defining Substrate Specificities for Lipase and Phospholipase Candidates

Defining Substrate Specificities for Lipase and Phospholipase Candidates

DOI:
10.3791/54613-v

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08:59 min

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November 23, 2016

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, , , ,
1Centro de Ciencias Genómicas,Universidad Nacional Autónoma de México

Capitoli

  • 00:05Titolo
  • 01:02Use Artificial Substrates for Optimizing Enzyme Activities of (Phospho)lipases
  • 02:20In Vivo Identification of the Physiological Substrate of a Lipase
  • 05:48In Vitro Identification of the Physiological Substrate of Diacylglycerol (DAG) Lipase
  • 07:00Results: Predicted Phospholipase SMc01003 Acts on Artificial p-Nitrophenyl Acyl Ester and DAG
  • 08:01Conclusion

Summary

Traduzione automatica

Traduzione automatica

Many predicted (phospho)lipases are poorly characterized with regard to their substrate specificities and physiological functions. Here we provide a protocol to optimize enzyme activities, search for natural substrates, and propose physiological functions for these enzymes.

Tags

Keywords: LipasePhospholipaseSubstrate SpecificityLipid RemodelingCell-free Protein ExtractEnzyme AssayPara-nitrophenolRadiolabelingAcetate

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