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Definindo substrato Especificidades para a lipase e fosfolipase candidatos
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Biochemistry
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JoVE Journal Biochemistry
Defining Substrate Specificities for Lipase and Phospholipase Candidates

Definindo substrato Especificidades para a lipase e fosfolipase candidatos

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14,732 Views

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08:59 min

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November 23, 2016

DOI:

10.3791/54613-v

DOI:
10.3791/54613-v

•

08:59 min
November 23, 2016

•

3 Views
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1Centro de Ciencias Genómicas,Universidad Nacional Autónoma de México

Summary

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Many predicted (phospho)lipases are poorly characterized with regard to their substrate specificities and physiological functions. Here we provide a protocol to optimize enzyme activities, search for natural substrates, and propose physiological functions for these enzymes.

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Sahonero-Canavesi, D. X., Zavaleta-Pastor, M., Martínez-Aguilar, L., López-Lara, I. M., Geiger, O. Defining Substrate Specificities for Lipase and Phospholipase Candidates. J. Vis. Exp. (117), e54613, doi:10.3791/54613 (2016).

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