Rivista
/
Chimica
/
Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds
JoVE Journal
Chimica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Chimica
Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds

Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds

9,714 Views

•

10:13 min

•

November 12, 2016

DOI:

10.3791/54611-v

DOI:
10.3791/54611-v

•

10:13 min
November 12, 2016

•

5 Views
, ,
1Center for Applied Plant Sciences,The Ohio State University, OARDC, 2Department of Plant Pathology,The Ohio State University, OARDC, 3Department of Horticulture and Crop Science,The Ohio State University, OARDC, 4OARDC Metabolite Analysis Cluster,The Ohio State University, OARDC

Summary

Automatically generated

This article presents a method for the detection and quantification of organic acids from plant material using free zonal capillary electrophoresis. An example of the potential application of this method, determining the effects of a secondary fermentation on organic acid levels in coffee seeds, is provided.

Explore More Videos

Capillary ElectrophoresisOrganic AcidsCoffea ArabicaCoffee SeedsShort Chain Carboxylic Acids (SCCAs)Sample PreparationLiquid Nitrogen GrindingAuthentic StandardsExternal And Internal StandardsCitric AcidMalic AcidAcetic AcidLactic AcidAdipic Acid

Read Article

Cite this Article

Vaughan, M. J., Chanon, A., Blakeslee, J. J. Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds. J. Vis. Exp. (117), e54611, doi:10.3791/54611 (2016).

Download .ris file

Copy

Share Video

.

Copy