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El uso de Inducida somática Análisis del Sector (ISSA) para el estudio de genes y promotores implicados en la formación de la madera y el desarrollo del tallo Secundaria
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Genetics
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JoVE Journal Genetics
The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development
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El uso de Inducida somática Análisis del Sector (ISSA) para el estudio de genes y promotores implicados en la formación de la madera y el desarrollo del tallo Secundaria

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8,664 Views

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09:54 min

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October 05, 2016

DOI:

10.3791/54553-v

DOI:
10.3791/54553-v

•

09:54 min
October 05, 2016

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1School of Ecosystem and Forest Sciences, Faculty of Science,The University of Melbourne, 2Victorian AgriBiosciences Centre,La Trobe University R&D Park, 3College of Biological Sciences, Department of Plant Biology,University of California, Davis, 4Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI),University of Pretoria

Summary

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Here we present a protocol that facilitates the medium to high throughput functional characterization of gene and promoter constructs in tree secondary stem tissue within comparatively short time frames. It is efficient, easy to use and widely applicable to a range of tree species.

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Spokevicius, A., Taylor, L., Melder, E., Van Beveren, K., Tibbits, J., Creux, N., Bossinger, G. The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development. J. Vis. Exp. (116), e54553, doi:10.3791/54553 (2016).

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