Journal
/
Genetics
/
L'utilisation de la Induced somatique analyse sectorielle (AISS) pour l'étude des gènes et de promoteurs impliqués dans la formation du bois et le développement de souches secondaire
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development
Please note that all translations are automatically generated. Click here for the English version.

L'utilisation de la Induced somatique analyse sectorielle (AISS) pour l'étude des gènes et de promoteurs impliqués dans la formation du bois et le développement de souches secondaire

français

Automatically Generated

8,664 Views

•

09:54 min

•

October 05, 2016

DOI:

10.3791/54553-v

DOI:
10.3791/54553-v

•

09:54 min
October 05, 2016

•

2 Views
, , , , , ,
1School of Ecosystem and Forest Sciences, Faculty of Science,The University of Melbourne, 2Victorian AgriBiosciences Centre,La Trobe University R&D Park, 3College of Biological Sciences, Department of Plant Biology,University of California, Davis, 4Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI),University of Pretoria

Summary

Automatically generated

Here we present a protocol that facilitates the medium to high throughput functional characterization of gene and promoter constructs in tree secondary stem tissue within comparatively short time frames. It is efficient, easy to use and widely applicable to a range of tree species.

Explore More Videos

Induced Somatic Sector AnalysisISSAWood FormationSecondary Stem DevelopmentGene CharacterizationPromoter AnalysisA.tumefaciensCambial WindowLB MediumMS MediumXylemPhloemHigh Throughput TechniqueTree Species

Read Article

Cite this Article

Spokevicius, A., Taylor, L., Melder, E., Van Beveren, K., Tibbits, J., Creux, N., Bossinger, G. The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development. J. Vis. Exp. (116), e54553, doi:10.3791/54553 (2016).

Download .ris file

Copy

Share Video

.

Copy