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The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development
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JoVE 杂志 遗传学
The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development

The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development

DOI:
10.3791/54553-v

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09:54 min

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October 05, 2016

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, , , , , ,
1School of Ecosystem and Forest Sciences, Faculty of Science,The University of Melbourne, 2Victorian AgriBiosciences Centre,La Trobe University R&D Park, 3College of Biological Sciences, Department of Plant Biology,University of California, Davis, 4Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI),University of Pretoria

Chapters

  • 00:05Title
  • 00:46Preparation of Agrobacterium tumefaciens for Inoculation
  • 01:59Inoculation of Plant Stems with A. tumefaciens
  • 03:44Harvesting for GUS Histological Assay
  • 05:57Identification of Transgenic Tissue
  • 07:22Results: Successful Transformation of Stem Tissue Using the ISSA Method
  • 09:12Conclusion

Summary

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Here we present a protocol that facilitates the medium to high throughput functional characterization of gene and promoter constructs in tree secondary stem tissue within comparatively short time frames. It is efficient, easy to use and widely applicable to a range of tree species.

Tags

Induced Somatic Sector AnalysisISSAWood FormationSecondary Stem DevelopmentGene CharacterizationPromoter AnalysisA.tumefaciensCambial WindowLB MediumMS MediumXylemPhloemHigh Throughput TechniqueTree Species

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