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Methoden om Onderzoek de lymfeklier en Hemocytes in<em> Drosophila</em> Larven
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Developmental Biology
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JoVE Journal Developmental Biology
Methods to Examine the Lymph Gland and Hemocytes in Drosophila Larvae
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Methoden om Onderzoek de lymfeklier en Hemocytes in<em> Drosophila</em> Larven

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12,590 Views

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11:49 min

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November 28, 2016

DOI:

10.3791/54544-v

DOI:
10.3791/54544-v

•

11:49 min
November 28, 2016

•

12 Views
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1The Graduate School of Biomedical Sciences,The Icahn School of Medicine at Mount Sinai, 2Department of Oncological Sciences,The Icahn School of Medicine at Mount Sinai

Summary

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Drosophila en zoogdieren hematopoietische systemen hebben veel gemeenschappelijke kenmerken, waardoor Drosophila een aantrekkelijke genetische model om hematopoiese te bestuderen. Hier laten we dissectie en montage van grote larvale hematopoëtische organen voor immunohistochemie. We beschrijven ook werkwijzen om verschillende larvale hematopoietische compartimenten waaronder circulerende hemocytes sessiele kristalcellen testen.

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Cite this Article

Reimels, T. A., Pfleger, C. M. Methods to Examine the Lymph Gland and Hemocytes in Drosophila Larvae. J. Vis. Exp. (117), e54544, doi:10.3791/54544 (2016).

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