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JoVE Journal Developmental Biology
Methods to Examine the Lymph Gland and Hemocytes in Drosophila Larvae
 

Methods to Examine the Lymph Gland and Hemocytes in Drosophila Larvae

Article DOI: 10.3791/54544-v • 11:49 min • November 28th, 2016
November 28th, 2016 •
1,2, 1,2
1The Graduate School of Biomedical Sciences, The Icahn School of Medicine at Mount Sinai, 2Department of Oncological Sciences, The Icahn School of Medicine at Mount Sinai

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Drosophila and mammalian hematopoietic systems share many common features, making Drosophila an attractive genetic model to study hematopoiesis. Here we demonstrate dissection and mounting of the major larval hematopoietic organ for immunohistochemistry. We also describe methods to assay various larval hematopoietic compartments including circulating hemocytes and sessile crystal cells.

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Lymph Gland Hemocytes Drosophila Larvae Immunohistochemistry Hematopoiesis Genetic Alteration Environmental Alteration Hemolymph Collection Dissection Pad Forceps Pipette Hemocytometer Chamber Hemolymph Immunochemistry Cover Slip
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