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Developmental Biology

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Methods to Examine the Lymph Gland and Hemocytes in Drosophila Larvae
 

Methods to Examine the Lymph Gland and Hemocytes in Drosophila Larvae

Article DOI: 10.3791/54544-v 11:49 min November 28th, 2016
November 28th, 2016

章节

总结概括

Drosophila and mammalian hematopoietic systems share many common features, making Drosophila an attractive genetic model to study hematopoiesis. Here we demonstrate dissection and mounting of the major larval hematopoietic organ for immunohistochemistry. We also describe methods to assay various larval hematopoietic compartments including circulating hemocytes and sessile crystal cells.

Tags

Keywords: Lymph Gland Hemocytes Drosophila Larvae Hematopoietic System Immunohistochemistry Hemolymph Collection Hemocytometer Hemolymph Immunochemistry Fixation Permeabilization Primary Antibody
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