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Cryomilled memeli hücrelerinden protein kompleksi olan ele geçirme kapasitesini
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Protein Complex Affinity Capture from Cryomilled Mammalian Cells
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Cryomilled memeli hücrelerinden protein kompleksi olan ele geçirme kapasitesini

Türkçe

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14,496 Views

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10:37 min

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December 09, 2016

DOI:

10.3791/54518-v

DOI:
10.3791/54518-v

•

10:37 min
December 09, 2016

•

7 Views
, ,
1Laboratory of Cellular and Structural Biology,The Rockefeller University, 2Institute for Systems Genetics, Department of Biochemistry and Molecular Pharmacology,New York University School of Medicine

Summary

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Burada, bir kriyojenik sıcaklıkta katı hal öğütme ile memeli hücrelerin bozulması Elde edilen hücre tozu, bir hücre, ekstrenin üretilmesi ve antikor-bağlı mikron çaplı paramanyetik taneler üzerine afinite yakalama ile ilgili protein kompleksleri izole etmek için protokoller tarif eder.

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Cite this Article

LaCava, J., Jiang, H., Rout, M. P. Protein Complex Affinity Capture from Cryomilled Mammalian Cells. J. Vis. Exp. (118), e54518, doi:10.3791/54518 (2016).

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