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Protein Complex Affinity Capture von Cryomilled Mammalian Cells
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Protein Complex Affinity Capture from Cryomilled Mammalian Cells
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Protein Complex Affinity Capture von Cryomilled Mammalian Cells

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14,496 Views

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10:37 min

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December 09, 2016

DOI:

10.3791/54518-v

DOI:
10.3791/54518-v

•

10:37 min
December 09, 2016

•

7 Views
, ,
1Laboratory of Cellular and Structural Biology,The Rockefeller University, 2Institute for Systems Genetics, Department of Biochemistry and Molecular Pharmacology,New York University School of Medicine

Summary

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Hier beschreiben wir Protokolle Säugerzellen durch Solid-State-Fräsen bei einer kryogenen Temperatur zu stören, um einen Zellextrakt aus der resultierenden Zelle Pulver produzieren, und Proteinkomplexe von Interesse durch Affinitätseinfangen auf Antikörper-gekoppelten Mikrometer-Maßstab paramagnetischen Perlen zu isolieren.

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Cite this Article

LaCava, J., Jiang, H., Rout, M. P. Protein Complex Affinity Capture from Cryomilled Mammalian Cells. J. Vis. Exp. (118), e54518, doi:10.3791/54518 (2016).

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